Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes
Anannya Banerjee
IRDM Faculty centre, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, Narendrapur, Kolkata -700103
Sudipta Tripathi
Institute of Agricultural Science, University of Calcutta, 51/2 Hazra Road, Kolkata-700019
DOI: https://doi.org/10.36956/njas.v1i1.9
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Abstract
Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.Keywords: Chromium tolerant bacterium, MIC, Growth curve, 16S rDNA, phylogenetic analysis, SEM, EDX
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