Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes

Anannya Banerjee(IRDM Faculty centre, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, Narendrapur, Kolkata -700103)
Sudipta Tripathi(Institute of Agricultural Science, University of Calcutta, 51/2 Hazra Road, Kolkata-700019)

Article ID: 9


Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.


Chromium tolerant bacterium; MIC; Growth curve; 16S rDNA; phylogenetic analysis; SEM; EDX

Full Text:



Alam, M. Z., & Malik, A. (2008). Chromate resistance, transport and bioreduction by Exiguobacterium sp. ZM‐2 isolated from agricultural soil irrigated with tannery effluent. Journal of basic microbiology, 48(5), 416-420.

Aneja, K. R. (2003). Experiments in microbiology, plant pathology and biotechnology. New Age International.

Banerjee, A., Tripathi, S., Mukherjee, A. K., & Mukherjee, S. (2018). Characterization of Bantala tannery sludge and its vermicompost. IJCS, 6(6), 185-189.

Bera,S. (2013). Stink in the leather belt, Down To Earth.

Chaturvedi, M. K. (2011). Studies on chromate removal by chromium-resistant Bacillus sp. isolated from tannery effluent. Journal of Environmental Protection, 2(01), 76.

Das, S., Mishra, J., Das, S. K., Pandey, S., Rao, D. S., Chakraborty, A.&Thatoi, H. (2014). Investigation on mechanism of Cr (VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere, 96, 112-121.

Familec, S., &Wieczorek-Ciurowa, (2011) K. R. Y. S. T. Y. N. A. Waste from Leather Industry. Threats to the Environment.

Ghori, M. I., Iqbal, M. J., & Hameed, A. (2011). Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes. Brazilian Journal of Microbiology, 42(1), 22-29.

Narayani, M. (2012). Characteristics of a novel Acinetobacter sp. and its kinetics in hexavalent chromium bioreduction. Journal of microbiology and biotechnology, 22(5), 690-698.

Shakoori, F. R., Tabassum, S., Rehman, A., &Shakoori, A. R. (2010). Isolation and characterization of Cr6+ reducing bacteria and their potential use in bioremediation of chromium containing wastewater. Pakistan J. Zool, 42(6), 651-658.

Shekhar, S., Sundaramanickam, A., &Vijayansiva, G. (2014). Detoxification hexavalent chromium by potential chromate reducing bacteria isolated from turnery effluent. Am J Res Commun, 2(2), 205-16.



  • There are currently no refbacks.

Copyright © 2019 Anannya Banerjee, Sudipta Tripathi

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
: +65 65881289 :